mrfp lact c2 lactadherin c2 lact c2  (Addgene inc)

Journal: Journal of Virology

Article Title: Phosphatidylserine Lateral Organization Influences the Interaction of Influenza Virus Matrix Protein 1 with Lipid Membranes

doi: 10.1128/JVI.00267-17

Figure Lengend Snippet: The PS sensor mRFP-Lact-C2 in HEK cells forms multimers at the PM. (A to C) Representative confocal fluorescence microscopy images of HEK cells labeled with the PS analogue NBD-PS (green channel) (A) and expressing mRFP-Lact-C2 (red channel) (B). The merged channels are shown in panel C. (D) Representative intensity/brightness map for HEK cells expressing mRFP-Lact-C2. Pixel intensity represents the average measured fluorescence intensity over 100 frames. The color code represents brightness values calculated from the corresponding intensity images, expressed as numbers of photons per molecule per second. Higher brightness values (i.e., red) correspond to larger apparent multimers. The brightness value for a myr-palm mRFP monomer under the same experimental conditions was ∼2.5 × 103 photons/molecule/s. (E) Normalized brightness distribution (Bnorm) calculated from the PM selection of 15 cells in a representative experiment. The normalization was performed by using the measured brightness of the myr-palm mRFP construct under the same experimental conditions as a monomeric reference. All imaging was performed on three different days and at 23°C. Bars, 10 μm.

Article Snippet: A plasmid expressing a lactadherin fragment fused to mRFP (mRFP-Lact-C2) (Addgene, Cambridge, MA) was a gift from Sergio Grinstein ( 46 ).

Techniques: Fluorescence, Microscopy, Labeling, Expressing, Selection, Construct, Imaging